Plant Transcription Factor Database
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT1G74500.1
Common NameATBS1, bHLH135, BS1, EN67, F1M20.18, PRE3, TMO7
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 93aa    MW: 10580.9 Da    PI: 9.3967
Description activation-tagged BRI1(brassinosteroid-insensitive 1)-suppressor 1
Gene Model
Gene Model ID Type Source Coding Sequence
AT1G74500.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
          HLH 14 driNsafeeLrellPkas.kapskKlsKaeiLekAveYIksL 54
                 d+iN+ + +L++llP++  + +s K+s a +L+ +++YI++L
                 79**************55599*******************99 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5088811.712661IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474591.96E-102079IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:, basic helix-loop-helix (bHLH) domain
CDDcd000834.74E-52065No hitNo description
PfamPF000104.6E-72061IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009740Biological Processgibberellic acid mediated signaling pathway
GO:0009742Biological Processbrassinosteroid mediated signaling pathway
GO:0010086Biological Processembryonic root morphogenesis
GO:0040008Biological Processregulation of growth
GO:0005634Cellular Componentnucleus
GO:0005737Cellular Componentcytoplasm
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000045anatomyembryo root
PO:0020109anatomyembryo hypophysis
PO:0001185developmental stageplant embryo globular stage
Sequence ? help Back to Top
Protein Sequence    Length: 93 aa     Download sequence    Send to blast
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT1G74500-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: At the globular stage, expressed in cells adjacent to the hypophysis and at later embryonic stages, restricted to the future root stem cells. {ECO:0000269|PubMed:20220754}.
UniprotTISSUE SPECIFICITY: Expressed in root and shoot meristems, and young siliques. Low levels detected in all aerial tissues. {ECO:0000269|PubMed:16527868, ECO:0000269|PubMed:20023194, ECO:0000269|PubMed:22339648}.
Functional Description ? help Back to Top
Source Description
TAIREncodes a basic helixloophelix transcription factor that acts downstream of MP in root initiation. TMO7 protein moves to the hypophysis and to vascular cells, contributing to MP-dependent root formation. Promotes the correct definition of the hypophysis cell division plane.
UniProtAtypical and probable non DNA-binding bHLH transcription factor required for MONOPTEROS-dependent root initiation in embryo. Promotes the correct definition of the hypophysis cell division plane. Transcriptionally controlled by MONOPTEROS. Moves from its site of synthesis in pro-embryos cells into the hypophysis. Regulates brassinosteroid (BR) signaling by sequestering negative BR signaling components. May function as positive regulator of gibberellin signaling. May play a role in the regulation of light signaling and possibly auxin signaling. {ECO:0000269|PubMed:16527868, ECO:0000269|PubMed:20023194, ECO:0000269|PubMed:20220754, ECO:0000269|PubMed:22339648}.
Function -- GeneRIF ? help Back to Top
  1. Plants use atypical Basic helix-loop-helix (bHLH) proteins to regulate BR signaling.
    [PMID: 20023194]
  2. TARGET OF MP, TMO5 and TMO7 encode basic helix-loop-helix (bHLH) transcription factors that are expressed in the hypophysis-adjacent embryo cells, and are required and partially sufficient for MONOPTEROS-dependent root initiation.
    [PMID: 20220754]
  3. TMO7 is involved in the regulation of light signaling.
    [PMID: 22339648]
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Not induced by exogenous gibberellin. {ECO:0000269|PubMed:16527868}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Interaction ? help Back to Top
Source Intact With
IntActSearch Q9CA64
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT1G74500
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK2273951e-156AK227395.1 Arabidopsis thaliana mRNA for putative DNA-binding protein, complete cds, clone: RAFL14-05-N07.
GenBankAY0882861e-156AY088286.1 Arabidopsis thaliana clone 519 mRNA, complete sequence.
GenBankBT0030461e-156BT003046.1 Arabidopsis thaliana At1g74500 gene, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_177590.18e-59transcription factor bHLH135
SwissprotQ9CA641e-60PRE3_ARATH; Transcription factor PRE3
TrEMBLD7KS262e-58D7KS26_ARALL; Putative uncharacterized protein
TrEMBLR0GE952e-58R0GE95_9BRAS; Uncharacterized protein
STRINGAT1G74500.13e-58(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP18771240
Publications ? help Back to Top
  1. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
  2. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
  3. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  4. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
  5. Hoth S, et al.
    Monitoring genome-wide changes in gene expression in response to endogenous cytokinin reveals targets in Arabidopsis thaliana.
    FEBS Lett., 2003. 554(3): p. 373-80
  6. Czechowski T,Bari RP,Stitt M,Scheible WR,Udvardi MK
    Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes.
    Plant J., 2004. 38(2): p. 366-79
  7. Wang XJ,Cao XL,Hong Y
    Isolation and characterization of flower-specific transcripts in Acacia mangium.
    Tree Physiol., 2005. 25(2): p. 167-78
  8. Branco-Price C,Kawaguchi R,Ferreira RB,Bailey-Serres J
    Genome-wide analysis of transcript abundance and translation in Arabidopsis seedlings subjected to oxygen deprivation.
    Ann. Bot., 2005. 96(4): p. 647-60
  9. Lee S, et al.
    Overexpression of PRE1 and its homologous genes activates Gibberellin-dependent responses in Arabidopsis thaliana.
    Plant Cell Physiol., 2006. 47(5): p. 591-600
  10. Hyun Y,Lee I
    KIDARI, encoding a non-DNA Binding bHLH protein, represses light signal transduction in Arabidopsis thaliana.
    Plant Mol. Biol., 2006. 61(1-2): p. 283-96
  11. Cao D,Cheng H,Wu W,Soo HM,Peng J
    Gibberellin mobilizes distinct DELLA-dependent transcriptomes to regulate seed germination and floral development in Arabidopsis.
    Plant Physiol., 2006. 142(2): p. 509-25
  12. Zhang LY, et al.
    Antagonistic HLH/bHLH transcription factors mediate brassinosteroid regulation of cell elongation and plant development in rice and Arabidopsis.
    Plant Cell, 2009. 21(12): p. 3767-80
  13. Wang H, et al.
    Regulation of Arabidopsis brassinosteroid signaling by atypical basic helix-loop-helix proteins.
    Plant Cell, 2009. 21(12): p. 3781-91
  14. Schlereth A, et al.
    MONOPTEROS controls embryonic root initiation by regulating a mobile transcription factor.
    Nature, 2010. 464(7290): p. 913-6
  15. De Rybel B, et al.
    A versatile set of ligation-independent cloning vectors for functional studies in plants.
    Plant Physiol., 2011. 156(3): p. 1292-9
  16. Arabidopsis Interactome Mapping Consortium
    Evidence for network evolution in an Arabidopsis interactome map.
    Science, 2011. 333(6042): p. 601-7
  17. Koizumi K,Wu S,MacRae-Crerar A,Gallagher KL
    An essential protein that interacts with endosomes and promotes movement of the SHORT-ROOT transcription factor.
    Curr. Biol., 2011. 21(18): p. 1559-64
  18. Castelain M,Le Hir R,Bellini C
    The non-DNA-binding bHLH transcription factor PRE3/bHLH135/ATBS1/TMO7 is involved in the regulation of light signaling pathway in Arabidopsis.
    Physiol Plant, 2012. 145(3): p. 450-60
  19. Saiga S, et al.
    Control of embryonic meristem initiation in Arabidopsis by PHD-finger protein complexes.
    Development, 2012. 139(8): p. 1391-8
  20. Meinke DW
    A survey of dominant mutations in Arabidopsis thaliana.
    Trends Plant Sci., 2013. 18(2): p. 84-91
  21. Ikeda M,Fujiwara S,Mitsuda N,Ohme-Takagi M
    A triantagonistic basic helix-loop-helix system regulates cell elongation in Arabidopsis.
    Plant Cell, 2012. 24(11): p. 4483-97
  22. Ikeda M,Mitsuda N,Ohme-Takagi M
    ATBS1 INTERACTING FACTORs negatively regulate Arabidopsis cell elongation in the triantagonistic bHLH system.
    Plant Signal Behav, 2013. 8(3): p. e23448